Firefighters were alerted when a train was halted near Melton after striking and killing four cows which had wandered on to the track.
The incident, which happened at Lag Lane in Thorpe Arnold, prevented the train from continuing on its journey.
Inspectors from Network Rail attened the scene to check the condition of the vehicle.
A spokesperson for Leicestershire Fire and Rescue, which sent a heavy rescue crew and a Ford Range with technical rescue crew from the Southern Fire Station. said: “We found one cow impaled on the train and it was removed by the fire service.
“The carcass was removed from the line to enable the train to proceed. Three further cows were also deceased due to the impact from the incident.”
The call-out was at 9.41am on Saturday morning and firefighters left the scene at 2.58pm.
A Network Rail spokesperson said:” On Saturday, at around 8.30am, we received reports that a train had struck three cows.
“There was damage to the front of the train. Passengers who were on-board left the train at Melton station and normal service was resumed just after 11am.”
The organsation said it was difficult to prevent incidents like this because it was not practical or desirable to fence the network’s 20,000 miles of track.
The spokesperson said: “When animals get on to the railway it can lead to delays as it is not safe to run trains where there is a risk of collision with a large animal.”
The organisation did not want to comment on individual incidents such as the one at Thorpe Arnold.
But the spokesperson added: “We educate land owners about the dangers and disruption caused by animal incursions, emphasising the need to keep gates securely closed and encouraging them to use additional measures such as electric fencing.
“If the line is fenced, we may change the type of fencing depending on the animals on the adjacent land.”